EVCertis - EV Research Service

Our Capabilities

Comprehensive EV Services

From isolation to functional assessment, we provide end-to-end support for your Extracellular Vesicle research, ensuring high purity, accurate characterization, and compliance with the latest MISEV2023 guidelines.

Purification and Physical Characterization

Purification of EVs from tissues (milk, plants, blood) and cell culture (2D & 3D bioreactors) using Tangential Flow Filtration (TFF) & Size Exclusion Chromatography (SEC) with high yield and purity
Nanoparticle Tracking Analysis
Dynamic Light Scattering
Nanoflow Cytometry (NanoFCM)
Transmission Electron Microscopy

Purity, Sterility and Stability Testing

Particle-to-Protein Ratio (PPR) by NTA and BCA assay
Agarose Sterility Test
Endotoxin Test (LAL)
Mycoplasma PCR Test
EV recovery under different conditions (temperature, buffer, freeze-thaw cycles)

Biochemical / Molecular Profiling

Single-EV surface protein analysis by NanoFCM
EV marker characterization by Western blot
RNA sequencing
Proteomics
Lipidomics

In vitro Uptake Assays

EV labeling and repurification (Acoerela, MemGlow, CFSE dyes)
Uptake quantification using flow cytometry and imaging (live or fixed cells)
Primary cells: human/mouse fibroblasts, blood, skin, hair cells, patient derived organoids
Cell lines: lung, skin, breast, colon, leukemia, lymphoma cells
Human skin biopsy

In vitro Functional Assays

Viability, proliferation, apoptosis, migration assays
qPCR, Western blots and flow cytometry of biomarkers
ELISA of cytokines & proteins
Differentiation assays based on histochemistry & immunostaining
ROS, hemolysis, pigmentation, metabolic, wound healing assays

In Vivo Biodistribution and Functional Studies

Biodistribution study in wildtype mice and tumor-bearing mice (real-time or post-treatment)
Treatments of leukemia, lung, colon, breast, pancreatic cancer
Tissue harvesting and staining
Immunoprofiling using flow cytometry & sequencing
Toxicity study: blood cell count, biochemistry & cytokine analysis in mice and larger animals

EV Functionalisation

Exogenous loading of small molecules, dyes, plasmids, mRNAs, siRNAs, miRNAs, and other nucleic acid types.
Conjugation of peptides, proteins including antibodies to the EV surface using click chemistry.

Custom Services

Don't see exactly what you need? We offer tailored research support, custom assay development, and specialized consultancy for EV projects to fit your exact specifications.

Discuss Your Project

Our Methodology

Adhering to the Highest Scientific Standards

At EVCertis, we recognize that reproducibility and standardization are the cornerstones of successful biotechnology research. Our protocols are strictly aligned with the latest international guidelines.

MISEV 2023 Compliance

All isolation and characterization services follow the Minimal Information for Studies of Extracellular Vesicles guidelines.

Rigorous Quality Control

Every batch undergoes multi-parameter testing (NTA, protein assays, surface markers) before release.

Expert Consultation

Work directly with our team of specialized EV scientists to interpret data and plan downstream applications.

Data-Driven Results

Receive comprehensive, publication-ready reports detailing particle concentration, size distribution profiles, and biomarker validation for every service rendered.

Company

About EVCertis

With years of experience in EVs, we aim to provide standardised and reliable services for EV-related research.

We wish to support the wider scientific community in advancing extracellular vesicle knowledge, improving research reproducibility, and accelerate the development of meaningful discoveries in diagnostics, therapeutics, and biotechnology.

10+

Years Experience

40+

Publications

20+

Assay Types

5000+

Citations

EV Research Service